Semi-automated fluorometry of total estrogens in plasma during late pregnancy.
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چکیده
منابع مشابه
Semi-automated fluorometry of total estrogens in plasma during late pregnancy.
We describe a mechanized method for determining total estrogens in plasma during the last trimester of pregnancy. This method involves rapid enzymic hydrolysis, automatic extraction (in a tube) with cyclohexane/ethyl acetate, purification of the extracted material by anion-exchange chromatography on a disposable "mini-column" of Dowex AG1 X 2 (acetate form), and continuous-flow fluorometric qua...
متن کاملA semi-automated fluorometric method for total estrogens in pregnancy urine.
We report a continuous-flow fluorometric method for total urinary estrogens that involves the Kober reaction, with extraction of the reaction product into dichloroethane containing trichloroacetic acid as described by Hahnel and Jones [Clin. Chim. Acta 16, 185 (1967)]. The dichloroethane extraction gives greater stability to the Kober color and sharper separation of aqueous and organic phases. ...
متن کاملContribution of estriol to total urinary estrogens during pregnancy.
Estriol accounts for an average of 74% of the principal Kober-positive steroids in the urine of 13 women during uncomplicated pregnancies. This proportion is independent of the stage of pregnancy, from 20 weeks until term. The average ratio of estriol to ring-D a#{149}ketols (mainly 16ahydroxyestrone and 16-ketoestradiol-17) is about 5:1 over the same period. In 17 pregnant women with diabetes,...
متن کاملAutomated fluorometry as applied to the assay of individual estrogens in purified urinary extracts.
Using the Kober-lttrich fluorescence procedure, automated fluorometry was applied to the assayof individualestrogensand their 3-methyl ether derivatives in the purified urinary extracts of nonpregnant women. The values obtained by using this new automated scheme were comparedwith those obtained by the manual time-decay modification of the Slaunwhite-Engel fluorescence method; the comparisonshow...
متن کاملThe Renal Clearance of Endogenous Estrogens in Late Pregnancy.
Although several studies have been published describing the renal excretion of certain steroid hormones (2, 3), or groups of hormones and hormone metabolites (4-8), an analysis of the renal clearance of endogenous estrogens has not so far been described. This has been due principally to the lack of adequate chemical methods for the determination of estrogens in plasma. Methods for the determina...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1980
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/26.2.190